Technical Data
G3057-44G
GRP94 (Endoplasmin, 94kD Glucose-regulated Protein, GRP-94, Heat Shock Protein 90kD beta Member 1, Tumor Rejection Antigen 1, gp96 Homolog, HSP90B1, TRA1
Description:
GRP94, also known as Heat shock protein 90kD beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Recombinant human GRP94 protein, fused to His-tag at N-terminus.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.01-0.03ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-KEGVKFDESEKTKE from an internal region of HSP90B1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris buffered saline, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human HSP90B1. Species sequence homology: Canine, mouse, rat, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.