Technical Data
G3059
-Glucosidase, Almond
2500U
Molecular Biology Storage: 4CShipping: Blue Ice
Catalyzes the hydrolysis of glucosides. Emulsin originally was the term applied to the extract of sweet almonds. It remains as the historical prototype of -glucosidase. Many other sources of the enzyme have been described.

Emulsin also shows significant D-galactosidase activity and there is some question as to whether or not it is one enzyme with two activities, because non-parallel response to inhibitors points to two separate enzymes. D-mannosidase has also been reported in almond emulsin.

Source:
Almonds

Form:
Supplied as a light yellow to yellow tan powder.

Solubility:
Colorless to light yellow, clear to slightly hazy in water.

Solubility Concentration:
1.90-2.10mg/ml

Activity:
2.0u/mg

Unit Definition:
1 unit releases 1 mole of glucose per minute from salicin at 37C, pH 5.0

Assay Method:
Salicin is hydrolyzed by b-glucosidase to yield saligenen and b-D-glucose. The rate of formation of glucose is measured in a hexokinase/glucose-6-phosphate dehydrogenase system. One unit releases one micromole of glucose per minute at 37C and pH 5.0 under the specified conditions.

Specificity:
b-glucosides and b-galactosides are reportedly substrates for the enzyme.

Inhibitors:
HgCl2, other heavy metal ions, sulfhydryl-binding compounds and polyols.

Storage and Stability:
Lyophilized powder may be stored at 4C. Stable for 12 months at 4C. Reconstitute with sterile ddH2O. Store at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
9001-22-3

Molecular Weight:
66.5,117kD
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Anderson, F., Cunningham, W., and Manners, D.: Studies on Carbohydrate-Metabolizing Enzymes. 10. Barley [[beta]]-Glucosidases, Biochem. J., 90, 30 (1964). 2. Brady, R., O'Brien, J., Bradley, R., and Gal, A.: Sphingolipid Hydrolases in Brain Tissue of Patients with Generalized Gangliosidosis, Biochim. Biophys. Acta, 210, 193 (1970). 3. Chakrabarti, A., and Storey, K.: Enhanced Glucose Production from Cellulose Using Coimmobilized Cellulase and [[beta]]-Glucosidase, Appl. Biochem. Biotech., 22, 263 (1989). 4. Conchie, J., Gelman, A., and Levvy, G.: Inhibition of Glycosidases by Aldonolactones of Corresponding Configuration. The C-4- and C-6-Specificity of [[beta]]-Glucosidase and [[beta]]-Galactosidase, Biochem. J., 103, 609 (1967). 5. Dale, M., Ensley, H., Kern, K., Sastry, K., and Byers, L.: Reversible Inhibitors of [[beta]]-Glucosidase, Biochem., 24, 3530 (1985). 6. Dale, M., Kopfler, W., Chait, I., and Byers, L.: [[beta]]-Glucosidase: Substrate, Solvent, and Viscosity Variation as Probes of the Rate-Limiting Steps, Biochem., 25, 2522 (1986).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.