-Glucuronidase, Recombinant (D-Glucuronide glucuronosohydrolase, EC 18.104.22.168)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
Beta-Glucuronidase has been found in tissue extracts of mammals and other vertebrates, digestive juices of snails, molluscs, locusts, bacteria and plants. The enzyme catalyzes the hydrolysis of beta-glucuronides and also the transfer of glucuronly radicals to acceptor alcohols. beta-Glucuronidase is a structural protein of the endoplasmic reticulum and its occurance in the lysozomes is due to changes in the endoplasmic reticulum.
Beta-Glucuronidase catalyzes the following reaction:
beta-Glucuronidase beta-D-Glucuronide + H2O ---> Alcohol + D-Glucuronate
b-D-glucuronide + H2O --> D-glucuronate + ROH (R: alkyl group)
170u/mg protein (at 30°C and pH 7.1)
(percentage of rb-Glucuronidase activity)
Glucose-6-phosphate dehydrogenase (NADP+) 0.001%
Glucose-6-phosphate dehydrogenase (NAD+) 0.001%
NADPH oxidase 0.001%
NADH oxidase 0.001%
Alkaline phosphatase 0.001%
6.0-8.0 (1 week at 11°C)
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Recombinant b-D-Glucuronide glucuronosohydrolase expressed in Escherichia coli
Form: Supplied as a liquid in glycerol.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.