Technical Data
G3060-03
-Glucuronidase, Recombinant (D-Glucuronide glucuronosohydrolase, EC 3.2.1.31)
300U
Molecular Biology Storage: -20CShipping: Blue Ice
Beta-Glucuronidase has been found in tissue extracts of mammals and other vertebrates, digestive juices of snails, molluscs, locusts, bacteria and plants. The enzyme catalyzes the hydrolysis of beta-glucuronides and also the transfer of glucuronly radicals to acceptor alcohols. beta-Glucuronidase is a structural protein of the endoplasmic reticulum and its occurance in the lysozomes is due to changes in the endoplasmic reticulum.

Beta-Glucuronidase catalyzes the following reaction:
beta-Glucuronidase beta-D-Glucuronide + H2O ---> Alcohol + D-Glucuronate

Catalysis:
b-D-glucuronide + H2O --> D-glucuronate + ROH (R: alkyl group)

Activity:
1000u/ml

Specific Activity:
170u/mg protein (at 30C and pH 7.1)

Contaminants:
(percentage of rb-Glucuronidase activity)
Glucose-6-phosphate dehydrogenase (NADP+) 0.001%
Glucose-6-phosphate dehydrogenase (NAD+) 0.001%
NADPH oxidase 0.001%
NADH oxidase 0.001%
Alkaline phosphatase 0.001%

pH Stability:
6.0-8.0 (1 week at 11C)

pH Reactivity:
Optimum pH7.5

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
68kD
Source: Recombinant b-D-Glucuronide glucuronosohydrolase expressed in Escherichia coli
Purity: Purfied
Form: Supplied as a liquid in glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.