		Technical Data
G3500-13G	GluR2 (Glutamate Receptor)

Description:
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. Regulation of fast excitatory synaptic neurotransmission is mediated primarily by ionotropic glutamate receptors (iGluR)-ligand-gated cation (principally Ca2+) channels found on postsynaptic neurons throughout the central nervous system. iGluR receptors participate in neural development, plasticity, learning and memory, and are implicated in excitatory and neurodegenerative pathologies including stroke, Alzheimer’s disease, Huntington’s chorea and amyotrophic lateral sclerosis. Pharmacological properties and amino acid sequence similarities allow classification of iGluR into three main subfamilies named for their principal agonists: 1. NMDA receptors and the two non-NMDA receptor subfamilies 2. AMPA receptors and 3. kainate. Glutamate signaling also occurs via a family of G-protein coupled glutamate receptors referred to as the metabotropic glutamate receptors (mGluR). mGluRs can modulate excitatory synaptic transmission by regulating neurotransmitter release, influencing ion channel activity, and altering synaptic plasticity. Both ionotropic and metabotropic glutamate receptors are also present on macroglia (astrocytes and oligodendrocytes) where they have been shown to influence gene expression and may be involved in precursor cell proliferation and differentiation.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Immunogen): 0.5-5ug/ml
Immunohistochemistry (Human, mouse, rat, monkey): 5ug/ml
Western Blot (Human, rat, monkey): 1-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	3G156	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Fusion protein containing aa175-430 from the N-terminal region of GluR2.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes human GluR2 protein (~96kD). No crossreactivity with other AMPA/Kainate GluR subunits. Species Crossreactivity: mouse, rat and monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

US Biological application reference: Tucholski, J. et al., (2013) Schizophrenia Research, http://dx.doi.org/10.1016/j.schres.2013.01.031. 1. Vissavajjhala P., Exp. Neurology 142:296-312 (1996).