		Technical Data
G3900-01A	GLUT 1 (Glucose Transporter 1)

Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
GLUT 1 is a facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. It has a very broad substrate specificity and can transport a wide range of aldoses including pentoses and hexoses.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000; Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1:1000-1:5000; Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1). Not recommended to heat the samples prior to loading to prevent Glut protein aggregation.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
G3900-01D: GLUT 1, Mouse, Control Peptide (Glucose Transporter 1)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

Not determined

Immunogen:

12aa peptide sequence near the C-terminal, cytoplasmic domain of mouse brain glucose transporter-1 (Glut-1) (KLH). Cellular Localization: Cell membrane; Localizes primarily at the cell surface.

Purity:

Serum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins. Species Sequence Homology: 100% conserved in human, rat, rabbit, bovine and porcine; 90% conserved in chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Haspel, et al., J. Biol. Chem. 263: 398-403 (1986). 2. Birnbaum, et al., 83: 5784-5788 (1986). 3. Piper, et al., Am. J. Physiol. 260: C570-C580 (1991). 4. Harris, et al., Proc. Natl. Acad. Sci. 89: 7556-7560 (1992). 5. Baldwin, S.A., Biochim. Biophys. Acta 1154: 17-49 (1993). 6. Mueckler, M., Eur. J. Biochem. 219: 713-725 (1994).