Technical Data
G3900-01A
GLUT 1 (Glucose Transporter 1)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
GLUT 1 is a facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. It has a very broad substrate specificity and can transport a wide range of aldoses including pentoses and hexoses.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000
Western Blot: 1:1000-1:5000; Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1). Not recommended to heat the samples prior to loading to prevent Glut protein aggregation.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
12aa peptide sequence near the C-terminal, cytoplasmic domain of mouse GLUT 1, conjugated to KLH. Cellular Localization: Cell membrane; Localizes primarily at the cell surface. Species Sequence Homology: 100% conserved in human, rat, rabbit, bovine and po
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Haspel, et al., J. Biol. Chem. 263: 398-403 (1986). 2. Birnbaum, et al., 83: 5784-5788 (1986). 3. Piper, et al., Am. J. Physiol. 260: C570-C580 (1991). 4. Harris, et al., Proc. Natl. Acad. Sci. 89: 7556-7560 (1992). 5. Baldwin, S.A., Biochim. Biophys. Acta 1154: 17-49 (1993). 6. Mueckler, M., Eur. J. Biochem. 219: 713-725 (1994).