Technical Data
G3900-01C
GLUT 1 (Glucose Transporter 1)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family). Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, MW 40-60kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.

Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier. It is ubiquitously expressed and transport glucose in most cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL). Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1). Not recommended to heat the samples prior to loading to prevent Glut protein aggregation.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.

Optimal dilutions to be determined by the researcher.

Control Peptide: G3900-01D

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide consisting of 12aa sequence near the C-terminus of mouse/rat brain glucose transporter-1 (Glut-1) (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in PBS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins. Species sequence homology: human, rat, rabbit, bovine and porcine (100%); chicken (90%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Haspel et al., (1986) J. Biol. Chem. 263, 398-403. 2. Birnbaum, et al., (1986) 83, 5784-5788. 3. Piper et al., (1991) Am. J. Physiol. 260, C570-C580. 4. Harris et al. (1992) Proc. Natl. Acad. Sci. 89, 7556-7560. 5. Baldwin, SA (1993) Biochim. Biophys. Acta 1154, 17-49. 6. Mueckler, M (1994) Eur. J. Biochem. 219, 713-725.