Technical Data
G3900-03
GLUT 1 (Erythrocyte/brain HepG2 glucose transporter, Glucose transporter type 1, Glucose transporter type 1 erythrocyte/brain, GLUT, GLUTB, GT1, GTG1, Gtg3, SLC2A1, Solute carrier family 2 facilitated glucose transporter member 1)
Description:
Seven types of glucose transport carrier proteins designated as Glut 1-7 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60kD. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. Majority of the differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue-specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:12,000
Western Blot: 1:5000. Predicted MW is ~50kD.
Immunohistochemistry: 1:200
Immunoprecipitation: 2ul will immunoprecipitate 80-85% Glut 1 from rat hippocampal membranes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat hippocampal plasma membranes. The antibody labels glucose transporter (Glut 1) species from rat

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to [C]GLFHPLGADSQV, from human GLUT 1. Cellular Localization: Integral membrane protein. Localizes primarily at the cell surface (by similarity).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid, 0.02% sodium azide.
Specificity:
Recognizes human GLUT1. Species crossreactivity: rat. Does not react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. MInamishima YA et al., Blood 111(6):3236-44 (2008) 2. MInamishima YA et al., Mol. Cell. Biol. 28:5729-5741 (2009)