Technical Data
GLUT 1 (Erythrocyte/brain HepG2 glucose transporter, Glucose transporter type 1, Glucose transporter type 1 erythrocyte/brain, GLUT, GLUTB, GT1, GTG1, Gtg3, SLC2A1, Solute carrier family 2 facilitated glucose transporter member 1)
Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are seven types of glucose transport carrier proteins, designated as Glut 1 to 7. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60kD. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes, as in blood brain barrier and blood testis barriers.
GLUT1 is a major glucose transporter in the mammalian blood brain barrier. It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells.

Cellular Localization: Cell membrane

Positive Control: Human kidney membrane and human skeletal muscle.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: a band is seen at ~42kD on human kidney membrane prep, representing GLUT1 protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide made to an N-terminal region of the human GLUT1 protein (between residues 1-100). [Swiss-Prot #P11166]. Homology: 100% human and 93% mouse, rat, cow, and rabbit
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
This antibody is specific for GLUT1 protein. Species Crossreactivity: This antibody is reactive against the human protein. No other specie has been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hergovich, A., Lisztwan, J., Thoma, C. R., Wirbelauer, C., Barry, R. E., and Krek, W. (2006) Priming-Dependent Phosphorylation and Regulation of the Tumor Suppressor pVHL by Glycogen Synthase Kinase 3, 26, 5784-5796.