Technical Data
G3900-05A
GLUT 1 (Glucose Transporter 1)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins ( Glut-1 to Glut-7 , MW 40-60kD) and Sodium Glucose Co-Transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.

Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons. Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7 is expressed in liver and other gluconeogenic tissues and mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa, , monkey 621aa; ~ 50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue, and small intestine.


Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000. Identifies a major band at about 42-45kD
ELISA: 1:10,000-50,000. Control Peptide (G3900-05E) can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
ELISA: G3900-05E, GLUT 1, Control Peptide, Human

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide 13aa sequence near the C-terminus of human Glut-1 (KLH coupled).
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes GLUT 1. Does recognize Hep G2 type transporter and Glut-1 from Caco-2 cells. Species Crossreactivity: Human RBC, rabbit brain, mouse 3T3 L1 fibroblasts, rat brain adipocytes. Species Sequence Homology: Porcine, bovine, rabbit, mouse, rat: 100%; Chicken: 75%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mueckler, M., et al., Science 229: 941-945 (1985). 2. Haspel, et al., J. Biol. Chem. 263: 398-403 (1986). 3. Birnbaum, et al., (1986) PNAS 83: 5784-5788. 4. Piper, et al., Am. J. Physiol. 260: C570-C580 (1991). 5. Harris, et al., PNAS 89: 7556-7560 (1992). 6. Baldwin, S.A., BBA 1154: 17-49 (1993). 7. Mueckler, M., Eur. J. Biochem. 219: 713-725 (1994).