Technical Data
G3900-05K
GLUT 1 (Glucose Transporter 1)
Description:
Most mammalian cells transport glucose through a family of
membrane proteins known as glucose transporters. Molecular
cloning of these glucose transporters has identified a family of
closely related genes that encodes at least 7 proteins (Glut-1 to
Glut-13, Mol. Wt. 40-80kD) and sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue- specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml using ECL. Identifies a major band at about 42-45kD from purified rat brain membranes.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
G3900-05L: GLUT 1 (Glucose Transporter 1) Control Peptide

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide correesponding to 15aa near the C-terminus, cytoplasmic domain of human Glut-1 (KLH). Species Sequence Homology: porcine, bovine, rabbit, mouse and rat; 100%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human GLUT 1 (Glucose Transporter 1). Antibodies made to this sequence from this region have detected Glut-1 from human (RBC) and rabbit brain and mouse 3T3 L1 fibroblasts and rat brain adipocytes. It also recognizes the Hep G2 type transporter and Glut-1 from Caco-2 cells (3).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mueckler, M et al (1985) Science 229, 941-945; Haspel et al., (1986) J. Biol. Chem. 263, 398-403; Birnbaum, et al., (1986) 83, 5784-5788. 2. Piper et al., (1991) Am. J. Physiol. 260, C570-C580. 3. Harris et al. (1992) Proc. Natl. Acad. Sci. 89, 7556-7560.; see reviews by Baldwin, SA (1993) BBA 1154, 17-49; Mueckler, M (1994) Eur. J. Biochem. 219, 713-725.