Technical Data
G3900-12
GLUT 1 (Glucose Transporter 1)
Description:
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 5ug/ml, adipose and muscle tissue
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Peptide corresponding to 12aa, LFHPLGADSQV, of the C-terminus of human GLUT-1, conjugated to KLH.
Purity:
Purified by affinity chromatography on a Sepharose 4B CNBr linked peptide column.
Form
Supplied as a liquid in PBS, pH 7.1, 0.1% sodium azide.
Specificity:
Recognizes human glucose transporter 1 from muscle and adipose tissue. Does not crossreact with GLUT-4. The MW of the glucose transporter precipitated by the serum is ~46kD. Species Crossreactivity: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cloherty, E. et al. (2001). Sugar transporter regulation by ATP and quaternary structure. Blood Cells Mol Dis. 27:102-107.