Technical Data
G3900-15N
GLUT 2 (Glucose Transporter, Insulin Regulatable)
Description:
GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 0.1mg/ml; Use 10ul to label 1-2.5x10e5 cells in a total reaction volume 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Immunocytochemistry: 10ug/ml in fixed cells. Cells were fixed with PBS, 4% paraformaldehyde, 0.15% picric acid at RT for 20 minutes, and subsequently permeabilized and blocked with PBS, 0.1% Triton X-100, 1% BSA, 10% normal donkey serum at RT for 45 minutes. After blocking, cells were incubated with diluted G3900-15N overnight at 4°C followed by Rhodamine Red coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG2a5D1Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human GLUT 2-transfected NS0 cells.
Purity:
Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Form
Supplied as a liquid in PBS, 5% trehalose.
Specificity:
Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT 1 or GLUT 3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Olson, A.L. and Pessin, J.E., Annu. Rev. Nut. 16: 235-256 (1996). 2. Mahraoui, L., et al., J. Biochem. 298(3): 629-633 (1994).