Technical Data
G3900-15N
GLUT 2 (Glucose Transporter, Insulin Regulatable)
Description:
GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug to label 1x10e6 NTERA-2 human human cell line
Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (medulla), liver, normal pancreas array and pancreatic cancer tissue
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a5D1Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to aa1-524 from human GLUT 2 expressed in NS0 cells (P11168)
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, 5% trehalose.
Specificity:
Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT 1 or GLUT 3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Olson, A.L. and Pessin, J.E., Annu. Rev. Nut. 16: 235-256 (1996). 2. Mahraoui, L., et al., J. Biochem. 298(3): 629-633 (1994).