Technical Data
G3900-24C
GLUT 3 (Glucose Transporter)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa, , monkey 621aa;~ 50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue and small intestine. Glut-13 or proton myo-inositol transporter (HMIT; human 629aa, rat 618aa, ~75-90kD/67kD protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol. Rat HMIT is ~35% identical to rat GlutX1.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL). Detects a major band at ~45kD.
ELISA: Coat plates at 1ug/ml with Control Peptide.
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide:
G3900-24D: GLUT 3, Human (Glucose Transporter)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 12aa sequence in the C-terminus of human glucose transporter-3 (hGlut-3) (KLH).
Purity:
Purified by immunoaffinity chromatography.Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Synt
Form
Supplied as liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human GLUT 3 (Glucose Transporter). Species Crossreactivity: Does not recognize mouse/rat Glut-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kayano, T., et al., J. Biol. Chem. 263: 15245-15248 (1988). 2. Gould, et al., Diabetologia 35: 304-309 (1992). 3. Nagamatsu, et al., J. Biol. Chem. 267: 467-472 (1992). 4. Brant, et al., Biochem. Soc. Trans. 20: 235S-236S-403 (1992). 5. Baldwin, S.A., Biochim. Biophys. Acta 1154: 17-49(1993). 6. Mueckler, M., Eur. J. Biochem. 219: 713-725 (1994).