Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1:1000-1:5000 (ECL). ~45kD
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
G3900-27 GLUT 3, Rat Control Peptide (Glucose Transporter)
Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|50ul||4°C (-20°C Glycerol)||Blue Ice||Mouse||Rabbit|
| Not determined|
|A 12 AA C-terminal peptide sequence near the C-terminus of mouse/rat glucose transporter-3 (Glut-3) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. |
|Supplied as a liquid, neat serum, 40% glycerol.|
|Recognizes mouse GLUT 3 (Glucose Transporter). Species sequence homology: rat 100%. Does not recognize human Glut-3 because of significant interspecies differences in C-terminal Glut-3. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Gould et al., (1992) Diabetologia 35, 304-309; Nagamatsu et al., (1992) J. Biol. Chem. 267, 467-472; Brant et al, (1992) Biochem. Soc. Trans. 20, 235S-236S-403; see reviews by Baldwin, SA (1993) Biochim. Biophys. Acta 1154, 17-49; Mueckler, M (1994) Eur. J. Biochem. 219, 713-725.|