Technical Data
G3900-95A
GLUT 10 (Glucose Transporter)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.

Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-50,000; Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide: G3900-95 GLUT 10, Control Peptide, Human (Glucose Transporter)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A 16-AA peptide sequence near the cytoplasmic, C-terminus of Human Glut-11 (1) was coupled to KLH
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes human GLUT10. Human peptide sequence has no significant sequence homology with other gluts.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McVie-Wylie, et al., Genomics 72(1): 113-117 (2001). 2. Dawson, P.A., et al., Mol. Genet. Metab. 74: 186-199 (2001).