Technical Data
G3901-05
GLUT 13 (Glucose Transporter, Proton myo-Inositol Transporter, HMIT)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml and detected with antibodies.
Optimal dilutions to be determined by the researcher.

Control Peptide: G3901

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 16-AA peptide sequence near the cytoplasmic, C-terminus of Human Glut-13, coupled to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human GLUT13 (HMIT). Species Sequence Homology: rat: 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Uldry M et al ((2001) EMBO J. 20: 4467-4477.