Technical Data
G3990-01
Glutamate
Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: silver-intensified immunogold or fluorescence. Samples should be fixed with 0.1-2.5% glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative). The minimum glutaraldehyde concentration is 0.00%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
2000Tests-20CBlue IceRabbit
Concentration:
~2000 tests/ml
Immunogen:
Glutamate-glutaraldehyde
Purity:
Purified immunoglobulin
Form
Supplied as a liquid in 100mM phosphate buffer with 1% sterile goat serum, 0.05% thimerosal.
Specificity:
Recognizes Glutamate (L-Glutamate). Calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable crossreactivity (<1:100) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/Lglutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest crossreactivity (1:8) against free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue). Species Crossreactivity: all
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.