Technical Data
G3990-01A
Glutamate (BSA)
Description:
A distinct step in inter cellular communication involves termination of synaptic transmission via the removal of neurotransmitters by specialized transporters. The regulated exocytotoic release of neurotransmitters in response to neural activity requires storage within intracellular vesicles. In the nervous system, these vesicles are the synaptic vesicles that are derived from the endosomal compartment, whereas in endocrine cells larger secretory granules, such as the chromaffin granules of adrenal medulla, are derived from the trans golgi networks. Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells.

Applications:
Suitable for use in Dot Blot. Other applications not tested.

Recommended Dilution:
Dot Blot: 1:5,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
As reported
Immunogen:
L-Glutamic acid (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes Glutamate. No crossreactivity with L-aspartate, glutamine, asparagine, and alanine. Weak activity may be exhibited with Gly-Asp, GABA, beta-alanine, glycine and 5-aminovaleric acid at high concentration 5-10mM.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tanaka, K. (1993) Neurosci. Lett. 16:149. 2. Shashidharan, P. et al (1993) BBA 1216:161. 3. Rothstein, J. D. et al (1994) Neuron 13:713. 4. Rothstein, J. D. et al (1995) Ann Neurol 38: