Technical Data
G3990-03
Glutamate
Description:
Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins.

Applications:
Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:500-1:2500 using free floating sections by the PAP technique on rat hippocampus.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM2Q71Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatMouse
Concentration:
Not Determined
Immunogen:
Glutamate-Glutaraldehyde-BSA
Purity:
Purified
Form
Supplied as a liquid in PBS, 10mM sodium azide.
Specificity:
Recognizes rat Glutamate. The crossreactivities were determined using an ELISA test by competition experiments with the following compounds: L-Glutamate-G-BSA: 1; D-Glutamate-G-BSA: 1/100; L-Asparate-G-BSA: 1/50,000; GABA-G-BSA:1/50,000 (G=Glutaraldehyde, BSA=Bovine Serum Albumin)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chagnaud, J.L., et al., Brain Research 481: 175–181 (1989).