Technical Data
G4000-01M
GAD65 (Glutamate Decarboxylase 2, 65kD Glutamic Acid Decarboxylase, GAD-65, Glutamate Decarboxylase 65kD Isoform, GAD2)
Description:
Adults express two forms of glutamate decarboxylase (GAD), GAD65 and GAD67, which are encoded by different independently regulated genes. Both are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve endings; while GAD67 is more widely distributed throughout the cell. GAD65 preferentially synthesizes GABA for vesicular release and GAD67 preferentially synthesizes cytoplasmic GABA. GAD65 is predominantly expressed in pancreatic b cells and auto-antibodies to GAD65 are strongly associated with Insulin dependent diabetes mellitus (IMDM).

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminus of human GAD65.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human GAD65. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.