Technical Data
G4000-12M
GAD1 (Glutamate Decarboxylase 1, 67kD Glutamic Acid Decarboxylase, GAD-67, Glutamate Decarboxylase 67kD Isoform, GAD, GAD67)
Description:
Glutamic acid decarboxylase (GAD) catalyzes the conversion of L-glutamic acid to the inhibitory eurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, re-encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000 polypeptide, with 85aa residues, whereas human GAD67 encodes a Mr 67,000 polypeptide, with 594aa residues. GAD67 gene consists of 16 exons, spread over more than 45kb of genomic DNA. The AD67 gene contains an additional exon (exon 0) that together with part of exon 1, specifies the 5' ntranslated region of GAD67 mRNA. Human GAD67 shows 65% identity to GAD65 and is located in q31. GAD67 may play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to ead to pyridoxine dependency with seizures.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 5ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-100 of human GAD67 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human GAD1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.