Technical Data
G4000-20
Glutamate Decarboxylase 67 (GAD67)
Description:
Glutamic acid decarboxylase (GAD) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid
(GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% amino acid identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. GAD67 is cytoplasmic (594aa), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation or disease progression.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:10,000; Using ECL on rat and mouse brain lysate.
Immunohistochemistry (Paraffin): 1:500; No epitope retrieval necessary.
Optimal dilutions to be determined by the researcher.

Positive Control:
SKNSH cell lysate (human neuroblastoma), mouse cerebral cortex

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.


TypeIsotypeCloneGrade
MabIgG2a3H404Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat GAD67 protein
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes rat Glutamate Decarboxylase 67 at ~67kD. No detectable crossreactivity with GAD65 by Western Blot on rat brain lysate. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ladewig, J., et al., Stem Cells 26(7): 1705-1712 (2008). 2. King, A.E., et al., J. Comp. Neurol. 498: 277-294 (2006). 3. Ling, L.L., et al., Neuroscience 132: 1103-1113 (2005). 4. Watanabe, K., et al., Nature Neurosci. 8: 288-296 (2005). 5. Varea, E., et al., Neuroscience 136: 435-443 (2005).