Technical Data
G7121
Glutamine Synthetase
Description:
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45kD subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested

Recommended Dilution:
Western Blot: 1:500. Detected Glutamine Synthetase on 10ug of human brain lysates. Single band in sheep and rat brain
Immunohistochemistry: Rat brain tissue
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain tissue, rat brain cytosolic fraction, human brain lysate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q56Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceSheepMouse
Concentration:
~1mg/ml
Immunogen:
Glutamine synthetase purified from sheep brain.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.09% sodium azide, BSA.
Specificity:
Recognizes sheep Glutamine Synthase at ~45kD. Species Crossreactivity: human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J. Neurosci. Res. 28: 497–506 (1991). 2. Mol. Cell. Biol. 11: 5275–5284 (1991). 3. J. Neurochemistry 65: 2146–2156 (1995). 4. Experimental Neurology 139: 167–171 (1996). 5. J. Neurochemistry 66: 2050–2056 (1996). 6. Exp. Eye Res. 64: 519–527 (1997). 7. Weiner, I.D., et al., Gastroenterol. 124(5): 1432-1440 (2003).