Technical Data
G8123-01
Glutathione (NEM)
Description:
Glutathione is a tripeptide (gama-glutamylcysteinylglycine) molecule found in most mammmalian cells. It functions in basic metabolic processes such as serving as a sulfydryl buffer to maintain redox within the cell, participating in the transport of amino acids and detoxifying hydrogen and organic peroxides. Glutathione depletion can inhibit Th-1 associated cytokine production and favors a Th-2 associated immune response (3). Glutathione deficiency has also been linked to decreased patient survival in HIV-infected individuals. Overexpression of apoptotic protein Bcl-2 causes relocalization of glutathione from the cytoplasm into the nucleus where glutathione is thought to alter the redox and block caspase activity.

Applications:
Suitable for use in Flow Cytometry, ELISA
and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14E91Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Thiol-modified glutathione, conjugated to KLH.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF.
Specificity:
Recognizes glutathione conjugated to NEM. Species Crossreactivity: mammals
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Messina, J.P., Mazurkiewicz, J., Lawrence, D.A.: Anticarcinogenesis and Radiation Protection. (Eds. Cerutti, P.A., Nygaard, O.F., Simic, M.G.) Plenum Publishing Corp, pp. 407-412 (1987). 2. Stryer, L.,: Biochemistry. W.H. Freeman and Co., pp. 593-593 (1988). 3. Peterson, J.D., Herzenberg, L.A., Vasquez, K., Waltenbaugh, C., PNAS 95: 3071-3076 (1998). 4. Herzenberg, L.A., DeRosa, S.C., Dubs, J.G., Roederer, M., Anderson, M.T., Ela, S.W., Deresinski, S.C., Herzenberg, L.A., PNAS 94: 1967-1972 (1997). 5. Voehringer, D.W., McConkey, D.J., McDonnell, T.J., Brisbay, S., Meyn, R.E. PNAS 95: 2956-2960 (1998).