Technical Data
Glutathione (NEM)
Glutathione is a tripeptide (gama-glutamylcysteinylglycine) molecule found in most mammmalian cells. It functions in basic metabolic processes such as serving as a sulfydryl buffer to maintain redox within the cell, participating in the transport of amino acids and detoxifying hydrogen and organic peroxides. Glutathione depletion can inhibit Th-1 associated cytokine production and favors a Th-2 associated immune response (3). Glutathione deficiency has also been linked to decreased patient survival in HIV-infected individuals. Overexpression of apoptotic protein Bcl-2 causes relocalization of glutathione from the cytoplasm into the nucleus where glutathione is thought to alter the redox and block caspase activity.

Suitable for use in Flow Cytometry, ELISA
and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
25ug-20CBlue IceMouse
Thiol-modified glutathione, conjugated to KLH.
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF.
Recognizes glutathione conjugated to NEM. Species Crossreactivity: mammals
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Messina, J.P., Mazurkiewicz, J., Lawrence, D.A.: Anticarcinogenesis and Radiation Protection. (Eds. Cerutti, P.A., Nygaard, O.F., Simic, M.G.) Plenum Publishing Corp, pp. 407-412 (1987). 2. Stryer, L.,: Biochemistry. W.H. Freeman and Co., pp. 593-593 (1988). 3. Peterson, J.D., Herzenberg, L.A., Vasquez, K., Waltenbaugh, C., PNAS 95: 3071-3076 (1998). 4. Herzenberg, L.A., DeRosa, S.C., Dubs, J.G., Roederer, M., Anderson, M.T., Ela, S.W., Deresinski, S.C., Herzenberg, L.A., PNAS 94: 1967-1972 (1997). 5. Voehringer, D.W., McConkey, D.J., McDonnell, T.J., Brisbay, S., Meyn, R.E. PNAS 95: 2956-2960 (1998).