		Technical Data
G8127-11B	Glutathione Peroxidase 1 (GPX1, GPx-1, Cellular Glutathione Peroxidase, HGNC:4553, GSHPX1, GSHPx-1 MGC14399, MGC88245)

Description:
Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. It belongs to the glutathione peroxidase family.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and Paraffin): 1:2000- 1:20,000
Western Blot: 1:10,000- 1:100,000. Under reducing conditions recognizes a 22kD protein in human brain tissue and glutathione peroxidase isolated from human erythrocytes.
Optimal dilutions to be determined by the researcher.

Recommended Tissues:
Human and rat brain microglia.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Sheep

Concentration:

Not Determined

Immunogen:

A synthetic peptide, RRYSRRFQTIDIEPDIEALL corresponding to amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin. Cellular Localization: Cytoplasm

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute with 100ul of sterile dH2O.

Specificity:

Recognizes human glutathione peroxidase 1 (GPx-1) protein. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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