Technical Data
G8127-13
Glutathione Peroxidase 3 (GPX3, GSHPx-3, GPx-3, GSHPx-P, GPx-P, GPXP)
Description:
Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226aa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100kD homotetramer consisting of four identical 23kD subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry: 2-15ug/ml
Immunocytochemistry: 2-15ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-28K: IgG, H&L (HRP) Pab Rb xGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb xGt
I1904-28V: IgG, (HRP) Pab Rb xGt
I1904-29A: IgG, (HRP) Pab Rb xGt

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa74-226 of mouse GPX3 expressed in E. coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% sodium azide.
Specificity:
Recognizes endogenous mouse Glutathione Peroxidase 3 (GPX3). Species Crossreactivity: Human, rat. Does not crossreact with recombinant human GPX1 or GPX2 in Western Blots.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.