Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer.
Secreted in plasma. PTM: The N-terminus is blocked. Belongs to the glutathione peroxidase family.
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived |
|Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.|
|This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P). Species Crossreactivity: Human and rat, other species have not yet been ested.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Takahashi K, et al. J. Biochem. 108:145-148(1990). ; 2. Chu F.-F, et al. Blood 79:3233-3238(1992). ; 3. Yoshimura S, et al. Gene 145:293-297(1994). ; 4. Comhair S.A.A, et a. Am. J. Respir. Cell Mol. Biol. 23:350-354(2000). ; 5. Esworthy R.S, et al. Arch. Biochem. Biophys. 286:330-336(1991). ; 6. Fukuhara R, et al. Comp. Biochem. Physiol. 141B:428-436(2005). ; 7. Maser R.L, et al. J. Biol. Chem. 269:27066-27073(1994). ; 8. Schwaab V, et al. Gene 167:25-31(1995). ; 9. Martin-Alonso J.M, et al. J. Biochem. 114:284-291(1993). ; 10. Chu F.F, et al. Blood 79(12).: 3233-3238(1992).|