Technical Data
G8127-14G
Glutathione Peroxidase 4 (GPX4, GPX-4, GSHPx-4, Phospholipid Hydroperoxide Glutathione Peroxidase Mitochondrial, mtPHGPx, PHGPx, MGC103187, MGC118087, Phospholipid Hydroperoxidase, Sperm Nucleus Glutathione Peroxidase, snGPx, snPHGPx)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
Immunohistochemistry (paraffin): 0.3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Kidney and Testis lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CEEPLVIEKDLPHY, from the C-terminus of the protein sequence according to NP_002076.2; NP_001034937.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human GPX4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation. J Biol Chem. 2005 May 18; [Epub ahead of print] PMID: 15901730.