Technical Data
G8134-03
Glutathione-S-Transferase (Schistosoma japonicum) (Biotin)
Description:
GST (Glutathione S-Transferase) is a 26kD protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:50,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Glutathione-S-Transferase [Schistosoma japonicum]
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2,10 mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1m sterile dH2O.
Specificity:
Recognizes Glutathione-S-Transferase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.