Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:22,000
Western Blot: 1:1000-1:3000
Immunohistochemistry: 1:500-1:1500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | 4°C (-20°C Glycerol) | Blue Ice | | Goat |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Glutathione-S-Transferase [Schistosoma japonicum] |
| Purity: |
| Purified from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. |
| Form |
| Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with horseradish peroxidase (HRP). Reconstitution: Reconstitute with 1ml sterile dH2O or 40-50% glycerol for long term storage. |
| Specificity: |
| Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Crossreactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
|
|
