Technical Data
G8134-08
Glutathione-S-Transferase [Schistosoma japonicum] (TRITC)
Description:
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.

Applications:
Suitable for immunomicroscopy, ELISA and Western Blot. Other applications have not been tested.

Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444D)

Absorption Wavelength: 550nm Emission Wavelength: 570nm

Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Goat IgG

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
1.0 mg/ml
Immunogen:
Glutathione-S-Transferase [Schistosoma japonicum]
Purity:
Purified from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10 mg/ml BSA, IgG and Protease free, 0.01% Sodium Azide. Labeled with TRITC.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-STransferase Schistosoma japonicum].
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
J.A. Titus, et al. J. Immunol. Methods 50; 193, 1982.