Technical Data
G8135-36
Glutathione S-transferase M1 (GSTM1, MU, H-B, GST1, GTH4, GTM1, MU-1, GSTM1-1, MGC26563, GSTM1a-1a, GSTM1b-1b,Glutathione S-transferase M1, HB subunit 4, GST class-mu 1, Glutathione S-aryltransferase, Glutathione S-alkyltransferase, Glutathione S-transfer
Description:
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18L43Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
As reported
Immunogen:
Ni-NTA purified recombinant protein expressed in E. Coli strain M15.
Purity:
Purified
Form
As reported
Specificity:
Species Crossreactivity: N/A
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40.