Technical Data
G8167-01
Glycine Receptor, alpha1 (GLRA1, lycine receptor 48kD subunit, Glycine receptor strychnine-binding subunit, Glycine receptor subunit alpha-1, MGC138878, MGC138879, STHE)
Description:
Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48kD and 58kD, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Frozen): 1:1000
Optimal dilution determined by the researcher.

Positive control:
Cell extracts containing recombinant human glycine receptors and homogenates of rat spinal cord.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide from the amino terminus of the human alpha 1 glycine receptor subunit (ARSATKPMSPSDFLDKLMGC) (KLH). Cellular Localization: Cell membrane.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from ammonium bicarbonate. Reconstitute in 50ul sterile PBS.
Specificity:
Recognizes human Glycine Receptor, alpha1. Specific to the a1 and a2 subunits of the glycine receptor. Species Crossreactivity: Mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rajendra, S., et al. Pharmacol. Ther. 73: 121-146, 1997. 2. Raymond, L.A., et al. Nature. 361: 637-641, 1993.