The sialoglycoprotein designated “A” is more commonly known as Glycophorin A and is found on the membrane of cells of the erythroid lineage1.
Can be used for identifying and gating erythrocytes, erythroblasts and reticulocytes2 or as a marker for identifying leukemias of erythroid origin (AML-M6)3,4.
Flow cytometry: use 10ul direct from the vial per 1x10e7 erythrocytes or per 5x10e5 nucleated cells.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100 Tests||-20°C||Blue Ice||Human||Mouse|
|Human red blood cells followed by purified glycophorin A.|
|Purified from cell culture supernatant. Tested by electrophoresis and flow cytometry.|
|Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. PE conjugated.|
|Binds to glutaraldehyde fixed or neuraminidase treated erythrocytes but not to papain, trypsin or pronase treated erythrocytes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Langlois R.G et al., 1985. Flow cytometric characterization of normal and variant cells with monoclonal antibodies specific for Glycophorin A. J. Immunol. 134(6): 4009–4017. 2. Pattanapanyasat K. et al., 1994. Flow cytometric immuno-phenotyping of lymphocyte subsets in samples that contain a high proportion of non-lymphoid cells.Cytometry 18: 199–208. 3. Anderson L.C. et al., 1981. Glycophorin A and fetal hemoglobin as markers of early erythroid derivation in human leukemias. In Leukemia Markers ed. W. Knapp, Academic Press p 249–252. 4. Carey J.L. and Hanson CA 1993. Flow cytometric analysis of leukemia and lymphoma. In Flow Cytometry and Clinical Diagnosis ed D.F. Keren et al., ASCP Chicago p 203–226. 5. Anderson, S. E., et al., 1986. The inheritance of abnormal sialoglycoproteins found in a Gerbich negative individual. Pathology 18:407–412. |