Description:
The sialoglycoprotein designated “A” is more commonly known as Glycophorin A and is found on the membrane of cells of the erythroid lineage1.
Applications:
Can be used for identifying and gating erythrocytes, erythroblasts and reticulocytes2 or as a marker for identifying leukemias of erythroid origin (AML-M6)3,4.
Recomended Dilution:
Flow cytometry: use 10ul direct from the vial per 1x10e7 erythrocytes or per 5x10e5 nucleated cells.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 2Q1511 | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100 Tests | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~0.1mg/ml |
| Immunogen: |
| Human red blood cells followed by purified glycophorin A. |
| Purity: |
| Purified from cell culture supernatant. Tested by electrophoresis and flow cytometry. |
| Form |
| Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. PE conjugated. |
| Specificity: |
| Binds to glutaraldehyde fixed or neuraminidase treated erythrocytes but not to papain, trypsin or pronase treated erythrocytes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Langlois R.G et al., 1985. Flow cytometric characterization of normal and variant cells with monoclonal antibodies specific for Glycophorin A. J. Immunol. 134(6): 4009–4017. 2. Pattanapanyasat K. et al., 1994. Flow cytometric immuno-phenotyping of lymphocyte subsets in samples that contain a high proportion of non-lymphoid cells.Cytometry 18: 199–208. 3. Anderson L.C. et al., 1981. Glycophorin A and fetal hemoglobin as markers of early erythroid derivation in human leukemias. In Leukemia Markers ed. W. Knapp, Academic Press p 249–252. 4. Carey J.L. and Hanson CA 1993. Flow cytometric analysis of leukemia and lymphoma. In Flow Cytometry and Clinical Diagnosis ed D.F. Keren et al., ASCP Chicago p 203–226. 5. Anderson, S. E., et al., 1986. The inheritance of abnormal sialoglycoproteins found in a Gerbich negative individual. Pathology 18:407–412.
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