Technical Data
G8235-52D
Glypican 4, aa367-382 (K-glypican, UNQ474/PRO937, GPC4)
Description:
Glypican-4, previously known as K-glypican, is a GPI-linked cell surface heparan sulfate proteoglycan of the glypican gene family, that comprises 6 members at present. Its function is only partially understood at present. However, glypican-4 seems to be involved in the control of cell growth and diffe-rentiation, especially in the development of the central nervous system and the kidney. Furthermore, glypican-4 is expressed by haematopoetic stem cells and bone marrow stromal cells. Although the antiserum was prepared against a peptide from hu man glypican-4, due to the highly conserved structure and se-quence of glypican-4, antiserum anti-peptide 1 (aa88-101) was used successfully to study brain development in the mouse.

Applications:
Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:1000-1:2000
Immunohistochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
20ul-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic glypican-4 peptide (aa367-382, -RPHHPEERPTT-AAGTSL-) coupled to KLH (keyhole limpet haemocyanin)
Purity:
Serum
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Specificity:
Specific for a core protein epitope (aa 367-382) of human glypican- 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Siebertz B, Drzeniek Z, Stöcker G, Just U, Handt S, Haubeck HD (1999): Expression of glypican-4 in haematopoietic progenitor and bone marrow stromal cells. Biochem J 344: 937-43
2. Ford-Perriss M, Turner K, Guimond S, Apedaile A, Haubeck HD, Turnbull J, Murphy M (2003): Localisation of specific heparan sulfate proteoglycans during the proliferative phase of brain development. Dev Dyn 227: 170-184