Technical Data
G8252-02D
GNPDA1 (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060)
Description:
GNPDA1 is a 289aa cytoplasmic protein belonging to the glucosamine: galactosamine-6-phosphate isomerase family. It is one of the two mammalian glucosamine-6-phosphate deaminase enzymes. GNPDA1 is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium with an aldo:keto isomerization and an amination:deamination. GNPDA1 may trigger calcium oscillations in mammalian eggs at fertilization which in turn serve as an important factor for egg development and early embryo development. It is a homohexamer, ubiquitously expressed in most of human tissues with high expression in testis, ovary, placenta, and heart and also in various cell lines.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.50ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Spleen lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa250-289 of human GNPDA1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human GNPDA1. Species Crossreactivity: chimpanzee, equine, monkey, orangutan. Species sequence homology: feline, bovine, opossum, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weidanz, JA. et al. Brit. J. Haemat. 91:72-79 (1995). 2. Nakamura, Y. et al. Genomics. 68:179-186 (2000). 3. Arreola, R. et al. FEBS Lett. 551:63-70 (2003).