Technical Data
GNPDA1 (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060)
GNPDA1 is a 289aa cytoplasmic protein belonging to the glucosamine: galactosamine-6-phosphate isomerase family. It is one of the two mammalian glucosamine-6-phosphate deaminase enzymes. GNPDA1 is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium with an aldo:keto isomerization and an amination:deamination. GNPDA1 may trigger calcium oscillations in mammalian eggs at fertilization which in turn serve as an important factor for egg development and early embryo development. It is a homohexamer, ubiquitously expressed in most of human tissues with high expression in testis, ovary, placenta, and heart and also in various cell lines.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.50ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Spleen lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa250-289 of human GNPDA1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human GNPDA1. Species Crossreactivity: chimpanzee, equine, monkey, orangutan. Species sequence homology: feline, bovine, opossum, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weidanz, JA. et al. Brit. J. Haemat. 91:72-79 (1995). 2. Nakamura, Y. et al. Genomics. 68:179-186 (2000). 3. Arreola, R. et al. FEBS Lett. 551:63-70 (2003).