Technical Data
G8560-40
GOLGA8B (Golgin A8 Family Member B, Golgin Subfamily A Member 8B, GOLGA5, Golgin-67, KIAA0855)
Description:
Golgins are integral membrane proteins localized to the Golgi apparatus and participate in structural organization of Golgi. Golgin-67, a 67KD protein, is a novel member of this family. It consists of an N-teminal coiled-coil domain and a C-terminal transmembrane domain and structurally resembles Golgin-84. It consists of a putative Src phosphorylation motif and has been found to interact with Src, thereby mediating golgi organization. It might also have a role in docking:tethering of vesicles. It is the smallest member of a growing family of Golgi autoantigens. Autoantibodies to Golgin-67 have been identified in systemic rheumatic diseases.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa136-152 (EEKKANNEKQKAERELE) of human golgin-67.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human Golgin-67.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Eystathioy,T., Jakymiw,A., Fujita,D.J., Fritzler,M.J. and Chan,E.K. Human autoantibodies to a novel Golgi protein golgin-67: high similarity with golgin-95/gm 130 autoantigen J. Autoimmun. 14 (2), 179-187(2000). 2. Jakymiw,A., Raharjo,E., Rattner,J.B., Eystathioy,T., Chan,E.K. and Fujita,D.J. Identification and characterization of a novel Golgi protein, golgin-67 J. Biol. Chem. 275 (6), 4137-4144 (2000).