Technical Data
G8576-92B
GOLGA3 (Golgin Subfamily A Member 3, Golgi Complex-associated Protein of 170kD, GCP170, Golgin-160)
Description:
Golgi auto-antigen; probably involved in maintaining Golgi structure. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 0.1-0.3ug/ml, observed in human HeLa lysates on ~170kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-HSQSRASKEGPGE, from human GOLGA3, at C-terminal (NP_005886.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human GOLGA3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Clarke JH, Emson PC, Irvine RF. Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephron. Am J Physiol Renal Physiol. 2008 Nov;295(5):F1422-30.