Technical Data
G8577-50
Golgi Marker, 58K
Description:
58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and as a marker for the Golgi complex in Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:5000
Immunofluorescence: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot (Colometric): Whole rat liver extract.
Indirect Immunofluorescence: Cultured Chinese hamster ovary (CHO) cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13C140Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceRatMouse
Concentration:
Not Determined
Immunogen:
Golgi 58K full length native protein purified from rat liver. Cellular Localization: Cytoplasmic and Golgi apparatus.
Purity:
Ascites
Form
Supplied as a liquid in 15mM sodium azide.
Specificity:
Recognizes an epitope located on the rat microtubule-binding peripheral Golgi membrane 58K protein at 58kD. Species Crossreactivity: Canine, hamster, human. Species Sequence Homology: Bovine, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bloom, G.S. & Brashear, T.A., J. Biol. Chem. 264: 16,083 (1989). 2. Donaldson, J.G., et al., J. Cell Biol., 11: 2295 (1990). 3. Ktistakis, N.T., et al., J. Cell Biol. 113: 1009 (1991). 4. Azad, A.K., et al., J. Leukoc. Biol. 84: 1594-1603 (2008). 5. Missiaen, L., BBRC 294: 249-252 (2002).