Technical Data
Golgi Marker, 58K
58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and as a marker for the Golgi complex in Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:5000
Immunofluorescence: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot (Colometric): Whole rat liver extract.
Indirect Immunofluorescence: Cultured Chinese hamster ovary (CHO) cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceRatMouse
Not Determined
Golgi 58K full length native protein purified from rat liver. Cellular Localization: Cytoplasmic and Golgi apparatus.
Supplied as a liquid in 15mM sodium azide.
Recognizes an epitope located on the rat microtubule-binding peripheral Golgi membrane 58K protein at 58kD. Species Crossreactivity: Canine, hamster, human. Species Sequence Homology: Bovine, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bloom, G.S. & Brashear, T.A., J. Biol. Chem. 264: 16,083 (1989). 2. Donaldson, J.G., et al., J. Cell Biol., 11: 2295 (1990). 3. Ktistakis, N.T., et al., J. Cell Biol. 113: 1009 (1991). 4. Azad, A.K., et al., J. Leukoc. Biol. 84: 1594-1603 (2008). 5. Missiaen, L., BBRC 294: 249-252 (2002).