Technical Data
G8577-50D
Golgi protein 58K (Formiminotransferase cyclodeaminase, FTCD, Glutamate formiminotransferase, LCHC1)
Description:
58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Cellular Localization: Cytoplasmic and Golgi Apparatus

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.03-0.1ug/ml. Predicted molecular weight: 59kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: C-LREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648; NP_996848.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
Expected to recognize both reported isoforms. Species Crossreactivity: Reacts with Human. Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Chicken (84% identity with immunogen), Zebrafish (90% identity with immunogen) and porcine (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bloom GS & Brashear TA A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 264:16083-92 (1989).