The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain and mediates trafficking in the late endosome-bypass pathway. GPP130 continuously recycles between early Golgi and distal compartments.
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Western Blot: 1:1000.
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ug||4°C (-20°C Glycerol)||Blue Ice||Monkey||Mouse|
| 1mg/ml after reconstitution|
|GPP130 from Vero monkey cells.|
|Purified by affinity chromatography.|
|Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul sterile, PBS, 0.09% sodium azide.|
|Recognizes monkey GPP130. Cross-reacts with human GPP130.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein: A.D. Linstedt, et al.; Mol. Biol. Cell 8, 1073 (1997) 2. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol. 142, 377 (1998) 3. The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment: J. Klumperman, et al.; J. Cell Sci. 111, 3411 (1998) 4. Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004)|