Technical Data
G8581-25
GPP130 (Golgi Phosphoprotein of 130kD, GIMPc, cis-Golgi Integral Membrane Protein)
Description:
The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain and mediates trafficking in the late endosome-bypass pathway. GPP130 continuously recycles between early Golgi and distal compartments.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000.
Immunohistochemistry: Frozen sections.
Immunocytochemistry: 1:1000.
Immunoprecipitation: 2ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D892Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceMonkeyMouse
Concentration:
1mg/ml after reconstitution
Immunogen:
GPP130 from Vero monkey cells.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul sterile, PBS, 0.1% sodium azide.
Specificity:
Recognizes monkey GPP130. Cross-reacts with human GPP130.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein: A.D. Linstedt, et al.; Mol. Biol. Cell 8, 1073 (1997) 2. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol. 142, 377 (1998) 3. The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment: J. Klumperman, et al.; J. Cell Sci. 111, 3411 (1998) 4. Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004)