Technical Data
G8600-34F
G-Protein Coupled Receptor 30 (GPCR30, GPR30, CEPR, Chemoattractant Receptor-like 2, CMKRL2, DRY12, Flow-induced Endothelial G-protein Coupled Receptor 1, FEG-1, GPCR-BR, G-protein Coupled Estrogen Receptor 1, GPER, IL8-related Receptor DRY12, LERGU, LERG
Description:
GPR30 (G Protein-coupled Receptor 30), also known as G Protein-coupled estrogen Receptor 1 (GPER), is a member of G protein-coupled receptor family. It contains seven hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops. GPR30 transduces a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR30 is an orphan receptor and localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives. Activating GPR30 via estrogen results in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to the amino terminal of human GPR30 protein, conjugated to KLH (Swiss Prot: Q99527).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Specificity:
Recognizes endogenous levels of human GPR30 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.