Technical Data
G-Protein Coupled Receptor 49 (GPCR49, GPR49, Leucine-rich Repeat Containing G-protein Coupled Receptor 5, Lgr5)
G-protein coupled receptor GPR49 (also known as Leucine-rich repeat containing G-protein coupled receptor 5, Lgr5) is closely related to members of the glycoprotein hormone receptor subfamily. It is expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain. GPR49 is reported to possibly mark stem cells in multiple adult tissues and is over expressed in cancers. G protein-coupled receptor GPR49 is an Orphan-A GPCR with an unknown ligand. GPR49 has been reported to be expressed in brain, skeletal muscle, placenta and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries, and from human germ cell, uterus and brain (neuroblastoma) cancer libraries.

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Unsuitable for use in Immunofluorescence/ Immunocytochemistry Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:20
Immunohistochemistry (Paraffin): 1:100-1:250
Flow Cytometry: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Controls:
Fetal skeletal muscle lysate, human muscle tissue, human ovarian, carcinoma tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to residues in human GPR49
Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Recognizes human GPR49 at ~100kD. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McDonald, T., et al., Biochem. Biophys. Research Commun. 247(2): 266-270 (1998). 2. Barker, N., Nature. 449(7165): 1003-1007 (2007). 3. Tanese, K., et al., Am. J. Pathol. 173(3): 835-843 (2008). 4. McClanahan, T., et al., Cancer Biology & Therapy 5(4): 419-426 (2006).