Technical Data
GPR109B (G-protein Coupled Receptor 109B, G-protein Coupled Receptor HM74, G-protein Coupled Receptor HM74B, Niacin Receptor 2, Nicotinic Acid Receptor 2, HM74B, NIACR2)
GPR109B is a membrane protein belonging to family 1 of G protein-coupled receptor (GPCR) is homologous to GPR109A. GPR109B, a potential 7 transmembrane-spanning receptor, shows a significant homology to B2 bradykinin receptor and to thrombin receptor. GPR109B mediates the anti-lipolytic and lipid-lowering effect of nicotinic acid by acting as a nicotinic acid receptor and results in a G protein-mediated decrease in cAMP levels and hence are promising targets for future pharmacotherapy. It is known to show highest expression in spleen followed by lymphocytes and adipose tissue.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa1-50 of human GPR109B.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human GPR109B. Species Crossreactivity: chimpanzee. Species sequence homology: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tunaru, S. et al. Nat. Med. 9:352-355 (2003). 2. Nomura, H. et al. Int. Immunol. 5:1239-1249 (1993). 3. Wise, A. et al. J. Biol. Chem. 278:9869-9874 (2003).