Technical Data
G8750-02A
Gr-1 (GR1, Myeloid Differentiation Antigen, Ly-6G) (FITC)
Description:
Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein (1). In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow (2). In the periphery, Ly-6G is expressed predominantly on neutrophils (3, 4).

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot and In vitro activation of complement-dependent cytoxicity Assays. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells.
Immunohistochemistry: frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b,kRB6-8C5Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Normal mouse bone marrow cells.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with FITC.
Specificity:
Recognizes mouse Gr-1 at ~21-25kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399. 2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22. 3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313. 4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139. 5. Southern Biotechnology Associates, Inc. Unpublished observations. 6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819. 7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505. 8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151. 9. Tumpey, T.M., S.-H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898. 10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259. 11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836. 12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827.