Technical Data
G8950-10
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
5ug
20ug
Growth Factors, Cytokines Storage: -20CShipping: RT
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages. It promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Recombinant human GM-CSF is a 14.6kD globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Biological Activity:
GM-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg.

Responding Cells (Partial List):
Certain BM precursor cells

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Country of Origin:
USA

Molecular Weight:
14.6kD
Source: Human, recombinant, E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ ug (1EU/ug).
Form: Supplied as a sterile-filtered, lyophilized powder from 10mM sodium citrate, pH 3.5. No preservative added. Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. This solution can be diluted into other buffered solutions or stored at -20C for future use.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.