Technical Data
G8950-10E
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
2ug
10ug
100ug
Growth Factors, Cytokines Storage: -70CShipping: Blue Ice
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF) is a glycoprotein of MW 20kD containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

AA Sequence:
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Biological Activity: Recombinant Human Granulocyte-Colony Stimulating Factor is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is less then 0.1ng/ml, corresponding to a Specific Activity of 1x108IU/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.8kD
Source: Recombinant, human, E. coli
Purity: 95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Thrombocytopenia in association with splenomegaly during granulocyte-colony-stimulating factor treatment in mice is not caused by hypersplenism and is resolved spontaneously.
Transfusion 2007 Jan;47(1):41-9
2. Successful mobilization of peripheral blood stem cells by use of granulocyte-colony stimulating factor and their transcoronary transplantation in a patient after cardiogenic shock due to acute myocardial infarction. J Invasive Cardiol 2006 Dec;18(12):614-6
3. Ethnic differences in plasma levels of interleukin-8 (IL-8) and granulocyte colony stimulating factor (G-CSF). Transl Res 2007 Jan;149(1):10-4
4. Granulocyte colony-stimulating factor prevents progression of monocrotaline-induced pulmonary arterial hypertension in rats. Circ J 2007 Jan;71(1):138-43
5. Lung large cell carcinoma producing granulocyte-colony-stimulating factor. Ann Thorac Surg 2007 Jan;83(1):308-10. Granulocyte colony stimulating factor in patients with large acute myocardial infarction: results of a pilot dose-escalation randomized trial.
Am Heart J 2006 Dec;152(6):1051.e9-14

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.