Technical Data
Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
Molecular Biology Storage: -20CShipping: RT
A glycoprotein of MW 20kD containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

Recombinant Human G-CSF is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kD

E. coli

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

0.1ng/ug (1EU/ug)

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.

Biological Activity:
Recombinant Human Granulocyte-Colony Stimulating Factor is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is less then 0.1ng/ml, corresponding to a Specific Activity of 1x10e8 IU/mg.

Reconstitution: Reconstitute with sterile ddH2O, protein carrier to >100ug/ml.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder in 10mM sodium acetate buffer, pH 4.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Detection of free hepatitis C virus core antigen by enzyme-linked immunosorbent assay is not suitable for screening of granulocyte colony-stimulating factor-mobilized hematopoietic progenitor donors. Lozano ML, Candela MJ, Zuazu I, Transfusion 2004 Dec;44(12):1755-61 2. Effect of bovine granulocyte colony-stimulating factor on the development of pneumonia caused by Mannheimia haemolytica. Youssef SA, Clark ME, Vet Pathol 2004 Nov;41(6):649-57 3. Granulocyte--colony-stimulating factor (Filgrastim) may overcome imatinib-induced neutropenia in patients with chronic-phase myelogenous leukemia. Jorgensen HG, Copland M, Cancer 2005 Jan 1;103(1):210-11 4. Hepatocytes derived from peripheral blood stem cells of granulocyte-colony stimulating factor treated F344 rats in analbuminemic rat livers. Huiling X, Inagaki M, Ozaki A, J Surg Res 2004 Nov;122(1):75-82 5. Cooperative autocrine and paracrine functions of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in the progression of skin carcinoma cells. Obermueller E, Vosseler S, Mueller MM, Can cer Res 2004 Nov 1;64(21):7801-12 6. Squamous cell carcinoma of the cervix producing granulocyte colony-stimulating factor. Nasu K, Inoue C, Kashima K, Obstet Gynecol 2004 Nov;104(5 Pt 1):1086-8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.