		Technical Data
G8950-15C	Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)	10ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Human granulocyte colony-stimulating factor (G-CSF) is produced by recombinant DNA technology. In comparison with natural products, its bioactivity is similar in vivo & in vitro. rHuG-CSF is one of the main cytokines modulating the granulocytic hematopoiesis in bone marrow. It acts on the hematologic progenitor cells of granulocyte selectively, promoting its proliferation and differentiation. It enhances the function and counts of granulocyte in peripheral blood as well.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense

Source:
Escherichia coli.

Biological Activity:
Measured in a cell proliferation assay using NFS-60 cell line. Its specific activity is 1.0×10e8 IU/mg.

Endotoxin: 1EU/ug (LAL)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 1 month at 4 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18.2kD

Source: E. coli
Purity: 98% as determined by SDS-PAGE and HPLC.
Form: Supplied as a lyophilized powder from 0.2um sterile-filtered solution in PBS, pH 7.0, 50ug of HSA per 1ug of cytokine. Reconstitute with sterile, dH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.