Technical Data
G8950-15X
Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Human granulocyte colony-stimulating factor(hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molecular Weight: 18.8kD (175amino acids including N-terminal methionine)

Sequence:
MTPLGPASSL/ PQSFLLKCLE/ QVRKIQGDGA/ ALQEKLCATY/ KLCHPEELVL/ LGHSLGIPWA/ PLSSCPSQAL/ QLAGCLSQLH/ SGLFLYQGLL/ QALEGISPEL/ GPTLDTLQLD/ VADFATTIWQ/ QMEELGMAPA/ LQPTQGAMPA/ FASAFQRRAG/ GVLVASHLQS/ FLEVSYRVLR/ HLAQP

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.8kD
Source: Human, Recombinant E. coli
Purity: 95% by SDS PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Nomura H, et al.(1986) EMBO J , 5(5): 871-6 2. Nagata, S., et al.(1986) Nature, 319 : 415-418 3. Demetri, G. D., et al.(1991) Blood, 78: 2791-2808 4. Hill, C. P., et al (1993) Proc. Natl. Acad. Sci. USA, 90:5167-5171

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.